Florian C L, Pietsch T, Noble M, Williams S R
The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, UK.
Br J Cancer. 1997;75(7):1007-13. doi: 10.1038/bjc.1997.173.
Well-characterized cell lines established from primitive neuroectodermal tumours (PNETs) were examined by proton nuclear magnetic resonance (1H-NMR) spectroscopy and chromatographic analysis of perchloric acid extracts, following amplification in cell culture. A characteristic 1H-NMR spectroscopic metabolite pattern was found for medulloblastoma cell lines, which clearly discriminates these cells from PNETs of other locations in the central nervous system (CNS), on the basis of their N-acetyl aspartate (NAA) and aspartate expression. Medulloblastoma cell lines were heterogeneous in respect of their metabolite expression, possibly owing to the heterogeneity in their differentiation along lineages of the CNS. All PNET spectra displayed similar features, including decreased NAA and creatine peaks and increased signals from choline compounds (Cho) compared with normal cerebellum. The expression of NAA by the medulloblastoma lines was in the opposite order to the extent of neuronal differentiation, which may indicate their origin from a progenitor cell with the phenotype of an oligodendrocyte-type-2 astrocyte cell.
对从原始神经外胚层肿瘤（PNETs）建立的特征明确的细胞系进行细胞培养扩增后，通过质子核磁共振（1H-NMR）光谱和高氯酸提取物的色谱分析进行检测。基于N-乙酰天门冬氨酸（NAA）和天门冬氨酸的表达，发现髓母细胞瘤细胞系具有特征性的1H-NMR光谱代谢物模式，这可将这些细胞与中枢神经系统（CNS）其他部位的PNETs明确区分开来。髓母细胞瘤细胞系在代谢物表达方面存在异质性，这可能归因于其沿CNS谱系分化的异质性。与正常小脑相比，所有PNET光谱均显示出相似的特征，包括NAA和肌酸峰降低以及胆碱化合物（Cho）信号增加。髓母细胞瘤细胞系中NAA的表达与神经元分化程度呈相反顺序，这可能表明它们起源于具有少突胶质细胞-2型星形胶质细胞表型的祖细胞。
