Armour A, Mairs R J, Gaze M N, Wheldon T E
University of Glasgow, Department of Radiation Oncology, Cancer Research Campaign Beatson Laboratories, UK.
Br J Cancer. 1994 Sep;70(3):445-8. doi: 10.1038/bjc.1994.325.
Successful imaging or treatment of neuroblastoma with 131I-meta-iodobenzylguanidine (131I-mIBG) depends on the selectivity of active (type 1) uptake of mIBG in neuroblastoma cells relative to passive (type 2) uptake present in most normal tissues. This study investigates the effects of moderately elevated temperature (39-41 degrees C) on the cellular uptake of 131I-mIBG in two neuroblastoma cell lines [SK-N-BE(2c) and IMR-32] and in a non-neuronal (ovarian carcinoma) cell line (A2780). In SK-N-BE(2c), a cell line with high active uptake capacity, the specific (type 1) uptake was reduced by 75% (P < 0.001) at 39 degrees C. Both IMR-32 and A2780 have a low capacity for accumulation of mIBG by active uptake. These cell lines demonstrated a statistically significant increase in accumulation at 39 degrees C, mainly as a result of increased non-specific transport. At 41 degrees C uptake of 131I-mIBG was reduced in all cell lines. Thus, the active component of mIBG uptake is more vulnerable to increased temperature than the passive component. It seems probable that moderately increased temperature will have an unfavourable effect on the therapeutic differential for targeted radiotherapy of neuroblastoma using radiolabelled mIBG.
用¹³¹I-间碘苄胍（¹³¹I-mIBG）成功对神经母细胞瘤进行成像或治疗，取决于神经母细胞瘤细胞中mIBG的主动（1型）摄取相对于大多数正常组织中存在的被动（2型）摄取的选择性。本研究调查了适度升高的温度（39 - 41摄氏度）对两种神经母细胞瘤细胞系[SK-N-BE(2c)和IMR-32]以及一种非神经元（卵巢癌）细胞系（A2780）中¹³¹I-mIBG细胞摄取的影响。在具有高主动摄取能力的SK-N-BE(2c)细胞系中，在39摄氏度时特异性（1型）摄取降低了75%（P < 0.001）。IMR-32和A2780通过主动摄取积累mIBG的能力都较低。这些细胞系在39摄氏度时积累量有统计学显著增加，主要是由于非特异性转运增加。在41摄氏度时，所有细胞系中¹³¹I-mIBG的摄取都减少。因此，mIBG摄取的主动成分比被动成分更容易受到温度升高的影响。似乎适度升高的温度可能会对使用放射性标记的mIBG进行神经母细胞瘤靶向放疗的治疗差异产生不利影响。
