Sawyer G J, Dalchau R, Fabre J W
Division of Cell and Molecular Biology, University of London, UK.
Transpl Immunol. 1993;1(1):77-81. doi: 10.1016/0966-3274(93)90063-e.
LEW (RT1(1)) rats were primed for indirect allorecognition of DA (RT1avl) MHC molecules by immunizing either with synthetic peptides corresponding to the polymorphic regions of the RT1.Aavl classical class I MHC molecule, or with the isolated, denatured chains of the RT1.A class I, RT1.B alpha class II and RT1.B beta class II MHC molecules of the DA strain. These primed LEW rats received DA kidney allografts and were treated after grafting with cyclosporin A. Unprimed LEW controls mount a vigorous rejection response to DA kidney allografts and produce a strong antibody response to DA class I MHC antigens. Both the rejection and the antibody responses are virtually completely suppressed by cyclosporin A treatment in these controls. Priming to indirect allorecognition of donor MHC antigens did not diminish the effectiveness of cyclosporin A in suppressing the acute rejection of DA kidney grafts, but cyclosporin A could not suppress the early antibody response to the grafts in the primed rats. This finding could be of importance in clinical transplantation, where antibody-mediated graft damage might play an important role in both acute vascular rejection and chronic rejection.
通过用对应于RT1.Aavl经典I类MHC分子多态性区域的合成肽免疫，或用DA品系的RT1.A I类、RT1.BαII类和RT1.BβII类MHC分子的分离、变性链免疫，使LEW (RT1(1))大鼠对DA (RT1avl) MHC分子进行间接同种异体识别致敏。这些致敏的LEW大鼠接受了DA肾移植，并在移植后用环孢素A治疗。未致敏的LEW对照对DA肾移植产生强烈的排斥反应，并对DA I类MHC抗原产生强烈的抗体反应。在这些对照中，环孢素A治疗几乎完全抑制了排斥反应和抗体反应。对供体MHC抗原的间接同种异体识别致敏并没有降低环孢素A抑制DA肾移植急性排斥反应的有效性，但环孢素A不能抑制致敏大鼠对移植的早期抗体反应。这一发现可能在临床移植中具有重要意义，在临床移植中，抗体介导的移植物损伤可能在急性血管排斥反应和慢性排斥反应中都起重要作用。
