Pertuiset B, Beckerich J M, Gaillardin C
Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, Institut National Agronomique, Thiverval-Grignon, France.
Curr Genet. 1995 Jan;27(2):123-30. doi: 10.1007/BF00313425.
Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a highly conserved sequence in the Rab family. Four different genes were isolated. One of them, RYL1, was shown to be essential for cell viability. RYL1p displayed a high similarity with and tight phylogenetic relationships to SEC4p. When placed under the control of the GAL10 promoter, RYL1 was able to specifically relieve the thermosensitivity of a sec4-8 mutant of Saccharomyces cerevisiae. Therefore, it is proposed that RYL1 is a functional homologue of the S. cerevisiae SEC4 gene and is involved in the fusion of secretory vesicles with the plasma membrane in the general protein secretion pathway.
Rab家族的小GTP结合蛋白参与真核细胞内的囊泡运输。用从Rab家族高度保守序列推导的寡核苷酸筛选解脂耶氏酵母的基因文库。分离出四个不同的基因。其中一个基因RYL1被证明对细胞活力至关重要。RYL1p与SEC4p具有高度相似性和紧密的系统发育关系。当置于GAL10启动子的控制下时，RYL1能够特异性缓解酿酒酵母sec4 - 8突变体的温度敏感性。因此，有人提出RYL1是酿酒酵母SEC4基因的功能同源物，并且在一般蛋白质分泌途径中参与分泌囊泡与质膜的融合。
