Sakurai H, Ohishi T, Amakasu H, Fukasawa T
Laboratory of Molecular Genetics, Keio University School of Medicine, Tokyo, Japan.
FEBS Lett. 1994 Sep 5;351(2):176-80. doi: 10.1016/0014-5793(94)80098-7.
The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomyces cerevisiae. We have recently shown that GAL11-encoded protein (Gal11p) enhances basal transcription from the CYC1 promoter in a cell-free transcription system [(1993) Proc. Natl. Acad. Sci. USA 90, 8382-8386]. Here we indicate that Gal11p stimulates basal transcription in a gene-specific manner in vitro. We further suggest that the mechanism underlying the transcriptional stimulation by Gal11p is distinct from that by DNA-bound activators, since Gal11p stimulated transcription in a reaction system where activators were unable to enhance transcription due to the lack of intermediary factors.
GAL11基因编码一种辅助转录因子，酿酒酵母中许多（即便不是全部）基因的充分表达都需要该因子。我们最近发现，GAL11编码的蛋白（Gal11p）在无细胞转录系统中增强了CYC1启动子的基础转录[（1993年）《美国国家科学院院刊》90, 8382 - 8386]。在此我们指出，Gal11p在体外以基因特异性方式刺激基础转录。我们进一步表明，Gal11p转录刺激的潜在机制与DNA结合激活因子的机制不同，因为在一个反应系统中，由于缺乏中间因子，激活因子无法增强转录，但Gal11p却能刺激转录。
