Inouye S, Tsuji F I
Marine Biology Research Division, University of California at San Diego, La Jolla 92093.
FEBS Lett. 1994 Sep 5;351(2):211-4. doi: 10.1016/0014-5793(94)00859-0.
Highly purified recombinant Aequorea green fluorescent protein is able to undergo a reversible oxidation-reduction reaction in the presence of molecular oxygen. In the oxidized form in near UV light, the protein is highly fluorescent, but when reduced with sodium dithionite, it becomes completely non-fluorescent. On exposure to molecular oxygen the reduced, non-fluorescent protein reverts to its original fluorescent state.
高度纯化的重组水母绿色荧光蛋白在分子氧存在的情况下能够发生可逆的氧化还原反应。在近紫外光下的氧化形式中，该蛋白具有高度荧光性，但用连二亚硫酸钠还原时，它会变得完全无荧光。暴露于分子氧时，还原的无荧光蛋白会恢复到其原始荧光状态。
