水母绿色荧光蛋白。该基因的表达及重组蛋白的荧光特性。
Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.
作者信息
Inouye S, Tsuji F I
机构信息
Marine Biology Research Division, Scripps Institution of Oceanography, University of California at San Diego, La Jolla 92093.
出版信息
FEBS Lett. 1994 Mar 21;341(2-3):277-80. doi: 10.1016/0014-5793(94)80472-9.
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.
水母绿色荧光蛋白的互补脱氧核糖核酸（cDNA）在大肠杆菌中的表达产生了一种融合蛋白，其荧光激发光谱和发射光谱与天然绿色荧光蛋白的光谱几乎相同。此外，当该蛋白溶液与水母发光蛋白和钙离子混合时，会发出动物体内发光特有的绿色ish发光，表明向该蛋白发生了无辐射能量转移。
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