孤立马氏管分泌液体取决于完整的细胞骨架。（你提供的原文中“tick salivary glands”有误，应该是“Malpighian tubules”，正确译文为：孤立马氏管的液体分泌取决于完整的细胞骨架。）

Fluid secretion by isolated tick salivary glands dependent on an intact cytoskeleton.

作者信息

Lamoreaux W J, Needham G R, Coons L B

机构信息

Department of Biology, Memphis State University, TN 38152.

出版信息

Int J Parasitol. 1994 Jul;24(4):563-7. doi: 10.1016/0020-7519(94)90148-1.

DOI:10.1016/0020-7519(94)90148-1

PMID:8082986

Abstract

Isolated salivary glands from female Dermacentor variabilis (Say) were pre-treated with either cytochalasin D or nocodazol, followed by stimulation with dopamine. Glands pre-treated with 1 microM-cytochalasin D did not secrete fluid; pre-treatment with 1 nM-cytochalasin D did not significantly reduce fluid secretion. Glands pre-treated with 1 microM-nocodazol had a significant reduction in fluid secretion (P = 0.003); 1 nM-nocodazol did not significantly affect fluid secretion. Ligation of the main salivary duct and pre-treatment with 1 microM-cytochalasin D significantly increased gland weight compared to the dopamine stimulated controls (P = 0.0412). Cytochalasin D-treated type III acini had a significantly larger mean diameter compared to the dopamine control (P = 0.0047). Glands treated with 1 mM-verapamil plus 10 microM-dopamine exhibited a significant decrease in fluid secretion (P = 0.017), and when ligated, had a significantly decreased weight compared to the controls (P = 0.0028).

摘要

从雌性变异革蜱（Say）分离出的唾液腺，先用细胞松弛素D或诺考达唑进行预处理，然后用多巴胺刺激。用1微摩尔/升细胞松弛素D预处理的腺体不分泌液体；用1纳摩尔/升细胞松弛素D预处理并未显著减少液体分泌。用1微摩尔/升诺考达唑预处理的腺体液体分泌显著减少（P = 0.003）；1纳摩尔/升诺考达唑对液体分泌无显著影响。与多巴胺刺激的对照组相比，结扎主唾液腺并用1微摩尔/升细胞松弛素D预处理显著增加了腺体重量（P = 0.0412）。与多巴胺对照组相比，经细胞松弛素D处理的III型腺泡平均直径显著更大（P = 0.0047）。用1毫摩尔/升维拉帕米加10微摩尔/升多巴胺处理的腺体液体分泌显著减少（P = 0.017），结扎后与对照组相比重量显著减轻（P = 0.0028）。