Caserta M, Camilloni G, Venditti S, Venditti P, Di Mauro E
Centro di Studio per gli Acidi Nucleici, CNR, Rome, Italy.
J Cell Biochem. 1994 May;55(1):93-7. doi: 10.1002/jcb.240550111.
Information in DNA is not limited to sequence information. Both local and global conformational parameters are pivotal to the interaction with a number of relevant proteins. The function of the major components of the transcription machinery (RNA polymerase II, DNA topoisomerase I, nucleosomes, the TATA-binding factor) is dependent on the topological status of the substrate DNA molecule. The topological requirements and the conformational consensus that dictate the rules for localization of nucleosomes and define the active sites for DNA topoisomerase I have been established; the reaction of DNA topoisomerase I is regulated by a topological feedback mechanism. The integrating function of the free energy of supercoiling in the transcription process and the regulatory role of DNA topoisomerase I are discussed.
DNA中的信息并不局限于序列信息。局部和全局构象参数对于与许多相关蛋白质的相互作用都至关重要。转录机制的主要成分（RNA聚合酶II、DNA拓扑异构酶I、核小体、TATA结合因子）的功能取决于底物DNA分子的拓扑状态。已经确立了决定核小体定位规则并定义DNA拓扑异构酶I活性位点的拓扑要求和构象共识；DNA拓扑异构酶I的反应受拓扑反馈机制调控。文中讨论了超螺旋自由能在转录过程中的整合功能以及DNA拓扑异构酶I的调节作用。
