Corraliza I M, Campo M L, Soler G, Modolell M
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
J Immunol Methods. 1994 Sep 14;174(1-2):231-5. doi: 10.1016/0022-1759(94)90027-2.
We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.
我们提出了一种对Schimke尿素测定方法的改进方法，作为一种用于测量活化巨噬细胞中精氨酸酶的有价值的微量方法。该方法具有以下优点：(a) 使用少量样品（每次测定约25,000个巨噬细胞）；(b) 不干扰活化巨噬细胞中也存在的其他相关代谢物，如瓜氨酸或精氨酸；(c) 可以使用底物精氨酸的饱和浓度；(d) 比Schimke方法更灵敏，能够检测低至0.02 μmol的少量尿素。
