Leenen P J, de Bruijn M F, Voerman J S, Campbell P A, van Ewijk W
Department of Immunology, Erasmus University, Rotterdam, Netherlands.
J Immunol Methods. 1994 Sep 14;174(1-2):5-19. doi: 10.1016/0022-1759(94)90005-1.
In this review, we present and discuss a selected panel of antibody-defined markers expressed during different stages of mouse macrophage development. We distinguish four categories of markers, which are characteristic of: (1) macrophage precursors and immature macrophages (ER-MP12, ER-MP20, ER-MP54, ER-MP58); (2) mature macrophages in general (F4/80, BM8, Mac-1, Mac-2, ER-BMDM1); (3) macrophage subsets (ER-HR3, ER-MP23, ER-TR9, Forssman antigen, MOMA-1, MOMA-2, Monts-4, SER-4), and (4) IFN-gamma-stimulated macrophages (H-2Ia, LFA-1, ICAM-1, 158.2, MBR-2, TM-2, TM-4, and TM-5). It should be noted that many of the markers in this last category are inducible by other stimuli as well. The rigid classification of markers into four separate groups should be regarded as a digitalization of a continuum, thus inevitably implicating a simplification of the complex phenotypic changes that occur during mononuclear phagocyte development. Nevertheless, the current selection of antibodies against markers for different developmental stages of macrophages constitutes an important tool for characterization of mouse macrophages which participate in various biological processes.
在本综述中，我们展示并讨论了一组选定的、在小鼠巨噬细胞发育不同阶段表达的抗体定义标志物。我们区分出四类标志物，它们分别具有以下特征：(1)巨噬细胞前体和未成熟巨噬细胞(ER-MP12、ER-MP20、ER-MP54、ER-MP58)；(2)一般的成熟巨噬细胞(F4/80、BM8、Mac-1、Mac-2、ER-BMDM1)；(3)巨噬细胞亚群(ER-HR3、ER-MP23、ER-TR9、福斯曼抗原、MOMA-1、MOMA-2、Monts-4、SER-4)，以及(4)γ干扰素刺激的巨噬细胞(H-2Ia、LFA-1、ICAM-1、158.2、MBR-2、TM-2、TM-4和TM-5)。应当指出，最后一类中的许多标志物也可被其他刺激诱导。将标志物严格分为四个独立的组应被视为一个连续统一体的数字化，因此不可避免地意味着对单核吞噬细胞发育过程中发生的复杂表型变化进行了简化。然而，目前针对巨噬细胞不同发育阶段标志物的抗体选择构成了表征参与各种生物学过程的小鼠巨噬细胞的重要工具。
