Crowe S M, Vardaxis N J, Kent S J, Maerz A L, Hewish M J, McGrath M S, Mills J
National Centre for HIV Virology Research/AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Melbourne, Australia.
J Leukoc Biol. 1994 Sep;56(3):318-27. doi: 10.1002/jlb.56.3.318.
HIV-1 infection of peripheral blood monocyte-derived macrophages (MDMs) is unrelated to the level of CD4 expression on the surface of the cell, is associated with considerable donor variability, causes minimal cytopathology, and results in peak viral antigen production after 2 weeks of infection. Phagocytosis of opsonized Candida albicans by MDMs infected in vitro with several strains of HIV was compared with that of uninfected cells from the same donors; the proportion of MDMs containing the fluorescein isothiocyanate-labeled yeast was determined by flow cytometry and phase contrast microscopy. The intracellular localization of C. albicans was confirmed by confocal microscopy. Using paired MDMs from nine donors, 81% of uninfected and 53% of HIV-infected MDMs phagocytosed C. albicans. In addition, the number of yeast per cell was significantly higher in uninfected MDMs than in HIV-infected cells (mean 6.1 versus 2.5). These findings may partially explain the high incidence of mucocutaneous candidiasis in HIV-infected patients with advanced disease.
外周血单核细胞来源的巨噬细胞（MDM）感染HIV-1与细胞表面CD4表达水平无关，存在显著的供体差异，引起的细胞病理学变化极小，且在感染2周后病毒抗原产量达到峰值。将体外感染几种HIV毒株的MDM对调理过的白色念珠菌的吞噬作用与来自相同供体的未感染细胞进行比较；通过流式细胞术和相差显微镜确定含有异硫氰酸荧光素标记酵母的MDM比例。通过共聚焦显微镜确认白色念珠菌的细胞内定位。使用来自9名供体的配对MDM，81%的未感染MDM和53%的HIV感染MDM吞噬了白色念珠菌。此外，未感染MDM中每个细胞的酵母数量显著高于HIV感染细胞（平均6.1对2.5）。这些发现可能部分解释了晚期HIV感染患者黏膜皮肤念珠菌病的高发病率。
