Bergey E J, Cho M I, Blumberg B M, Hammarskjöld M L, Rekosh D, Epstein L G, Levine M J
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214.
J Acquir Immune Defic Syndr (1988). 1994 Oct;7(10):995-1002.
Previous studies have suggested that salivary secretions may act as inhibitors of HIV-1 replication in vitro. This inhibitory activity was determined to be associated mainly with secretions obtained from the human submandibular-sublingual glands, and subsequent electron micrographs revealed the association of viral particles with the salivary sediment. Fractionation of human submandibular-sublingual (HSMSL) saliva by size-exclusion chromatography was initiated, and resulting fractions were tested for their ability to modulate the replication of HIV-1 using a plaque assay on HeLa CD4+ cell monolayers. Results indicated that the filtration-sensitive inhibitory activity was primarily associated with the mucin-rich fractions, and the inhibitory activity was found to reduce the number of infectious units by 75%. To determine the identity of the salivary components involved, adsorption experiments involving the interaction of HIV particles with immobilized salivary components were performed. Immunological counter staining revealed an interaction of HIV particles as well as recombinant gp120 with the lower-molecular-weight mucin. Electron microscopic examination of the mucin-rich fractions-HIV incubates revealed the aggregation of virus particles by salivary components. These results suggest that human salivary mucins may have a role in modulating the infectivity of HIV-1.
先前的研究表明，唾液分泌物在体外可能作为HIV-1复制的抑制剂。已确定这种抑制活性主要与从人类颌下腺-舌下腺获得的分泌物有关，随后的电子显微镜照片显示病毒颗粒与唾液沉淀物有关。开始通过尺寸排阻色谱法对人类颌下腺-舌下腺（HSMSL）唾液进行分级分离，并使用对HeLa CD4+细胞单层进行的空斑试验，检测所得级分调节HIV-1复制的能力。结果表明，对过滤敏感的抑制活性主要与富含粘蛋白的级分有关，并且发现该抑制活性可使感染单位数量减少75%。为了确定所涉及的唾液成分的身份，进行了涉及HIV颗粒与固定化唾液成分相互作用的吸附实验。免疫反向染色显示HIV颗粒以及重组gp120与低分子量粘蛋白之间存在相互作用。对富含粘蛋白的级分与HIV孵育物进行电子显微镜检查，发现唾液成分使病毒颗粒聚集。这些结果表明，人类唾液粘蛋白可能在调节HIV-1的感染性方面发挥作用。
