Chio C C, Ho M T, Huang C C, Jin Y T, Lin M T
Department of Surgery (Division of Neurosurgery), National Cheng Kung University Medical Center, Tainan City, Taiwan 704, ROC.
Neurosci Lett. 1994 Apr 25;171(1-2):5-8. doi: 10.1016/0304-3940(94)90590-8.
In vivo voltammetry (IVV) was used in rats with transient brain ischemia to measure changes in extracellular concentrations of dopamine (DA) and its metabolites in the corpus striatum. Striatal neuronal damage were also rated on a scale of 0-3 (0 = no damage; 3 = maximum cell loss). The striatal extracellular levels of DA and its metabolites increased by 12-fold during the 30 min of brain ischemia and returned to control values at 30 min after reperfusion. In another group treated with 4-5 ml of 10% human albumin intravenously infused 30 min before brain ischemia, both augmented striatal DA (and its metabolites) levels and striatal neuronal damages were reduced as compared to the ischemic control group (P < 0.05, unpaired Student's t-test). These results suggest that hypervolemic hemodilution protects the striatal neurons from ischemic injury by reducing the extracellular striatal DA release in rats.
采用体内伏安法（IVV）对短暂性脑缺血大鼠进行研究，以测量纹状体内多巴胺（DA）及其代谢产物的细胞外浓度变化。同时，根据0-3级评分标准对纹状体神经元损伤情况进行评估（0级 = 无损伤；3级 = 最大程度的细胞丢失）。在脑缺血的30分钟内，纹状体细胞外DA及其代谢产物水平升高了12倍，并在再灌注后30分钟恢复至对照值。在另一组实验中，于脑缺血前30分钟静脉输注4-5毫升10%人白蛋白，与缺血对照组相比，该组大鼠纹状体DA（及其代谢产物）水平升高以及纹状体神经元损伤均有所减轻（P < 0.05，非配对学生t检验）。这些结果表明，高容量血液稀释通过减少大鼠纹状体细胞外DA释放，保护纹状体神经元免受缺血性损伤。
