Schoeni J L, Doyle M P
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706.
Appl Environ Microbiol. 1994 Aug;60(8):2958-62. doi: 10.1128/aem.60.8.2958-2962.1994.
Challenging 1-day-old White Leghorn chicks perorally with 2.6 x 10(1) to 2.6 x 10(5) Escherichia coli O157:H7 bacteria per chick resulted in cecal colonization at all levels. Two of six chicks inoculated with only 2.6 x 10(1) E. coli O157:H7 bacteria carried 10(3) to 10(4) E. coli O157:H7 bacteria per g of cecal tissue when sacrificed 3 months postinoculation. E. coli O157:H7 colonization persisted at least 10 to 11 months when chicks were administered 10(8) E. coli O157:H7 bacteria. Eggs from five hens that were fecal shedders of E. coli O157:H7 until the termination of the study (10 to 11 months) were assayed for E. coli O157:H7. The organism was isolated from the shells of 14 of 101 (13.9%) eggs but not from the yolks and whites. Considering that chicks can be readily colonized by small populations of E. coli O157:H7 and continue to be long-term shedders, it is possible that chickens and hen eggs can serve as vehicles of this human pathogen.
给1日龄的白来航鸡雏鸡经口接种每只2.6×10¹至2.6×10⁵个大肠杆菌O157:H7细菌，结果在各个接种水平下盲肠均出现了定植。接种仅2.6×10¹个大肠杆菌O157:H7细菌的6只雏鸡中，有2只在接种后3个月处死时，每克盲肠组织中携带10³至10⁴个大肠杆菌O157:H7细菌。当给雏鸡接种10⁸个大肠杆菌O157:H7细菌时，大肠杆菌O157:H7的定植持续了至少10至11个月。对5只在研究结束时（10至11个月）一直作为大肠杆菌O157:H7粪便排出者的母鸡所产的鸡蛋进行了大肠杆菌O157:H7检测。在101枚鸡蛋中的14枚（13.9%）蛋壳中分离出了该菌，但在蛋黄和蛋清中未分离到。鉴于雏鸡能够很容易地被少量大肠杆菌O157:H7定植并持续成为长期排出者，鸡和鸡蛋有可能成为这种人类病原体的传播媒介。
