节杆菌属细菌通过1,2,4-苯三酚对对硝基苯酚的生物降解作用
Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.
作者信息
Jain R K, Dreisbach J H, Spain J C
机构信息
Armstrong Laboratory, Tyndall Air Force Base, Florida 32403-6001.
出版信息
Appl Environ Microbiol. 1994 Aug;60(8):3030-2. doi: 10.1128/aem.60.8.3030-3032.1994.
Abstract
The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP with stoichiometric release of nitrite. During induction of the enzymes required for growth on PNP, 1,2,4-benzenetriol was identified as an intermediate by gas chromatography-mass spectroscopy (GC-MS) and radiotracer studies. 1,2,4-Benzenetriol was converted to maleylacetic acid, which was further degraded by the beta-ketoadipate pathway. Conversion of PNP to 1,2,4-benzenetriol is catalyzed by a monooxygenase system in strain JS443 through the formation of 4-nitrocatechol, 4-nitroresorcinol, or both. Our results clearly indicate the existence of an alternative pathway for the biodegradation of PNP.
摘要
莫拉克斯氏菌属和假单胞菌属对硝基苯酚（PNP）的降解过程包括初始单加氧酶催化的硝基去除。生成的对苯二酚会受到双加氧酶催化的开环反应。我们分离出了一株节杆菌属菌株JS443，它能够降解PNP并按化学计量比释放亚硝酸盐。在诱导利用PNP生长所需的酶的过程中，通过气相色谱 - 质谱联用（GC - MS）和放射性示踪研究确定1,2,4 - 苯三酚为中间产物。1,2,4 - 苯三酚被转化为马来酰乙酸，后者通过β - 酮己二酸途径进一步降解。菌株JS443中的单加氧酶系统通过形成4 - 硝基邻苯二酚、4 - 硝基间苯二酚或两者将PNP转化为1,2,4 - 苯三酚。我们的结果清楚地表明存在PNP生物降解的替代途径。
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