Covello M, Mazzulla S, Covello C, Spena A, Marotta M, Martino G
Department of Cell Biology, University of Calabria, Rende, CS.
Boll Soc Ital Biol Sper. 1994 Apr;70(4):89-95.
In this research the amino acidic composition of a peptide fraction from mitochondrial DNA has been determined, using HPLC and the fingerprinting technique. This peptide fraction has been shown to be composed of two sub-fractions with different reactivity to ninhydrin, and thus supplied with several N-substitute groups. The composition of the first fraction is Gln, Glu, Pro, Leu. The second fraction composition is Pro, Glu or Gln, Asp or Asn, Ser.
在本研究中，利用高效液相色谱法（HPLC）和指纹图谱技术测定了线粒体DNA中一个肽段的氨基酸组成。该肽段已被证明由两个对茚三酮反应性不同的亚组分组成，因此含有多个N-取代基团。第一个组分的组成是谷氨酰胺、谷氨酸、脯氨酸、亮氨酸。第二个组分的组成是脯氨酸、谷氨酸或谷氨酰胺、天冬氨酸或天冬酰胺、丝氨酸。
