恶性疟原虫中的一种新型葡萄糖-6-磷酸脱氢酶：cDNA与蛋白质一级结构
A novel glucose-6-phosphate dehydrogenase in Plasmodium falciparum: cDNA and primary protein structure.
作者信息
Shahabuddin M, Rawlings D J, Kaslow D C
机构信息
Molecular Vaccine Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
出版信息
Biochim Biophys Acta. 1994 Sep 13;1219(1):191-4. doi: 10.1016/0167-4781(94)90269-0.
The structure of the parasite-encoded G6PD (PfG6PD) may provide clues about the relative protection against malaria in humans with glucose-6-phosphate dehydrogenase (G6PD) deficiency. We have cloned Pfg6pd cDNA encoding a predicted 856 amino acid residues polypeptide with a calculated molecular mass of > 94 kDa. The predicted amino acid sequence is highly homologous to G6PD from other organisms. Pfg6pd maps as a single or low copy number gene to chromosome 14. The unusually large N-terminus and the distance between the NADP-binding site and G6PD-binding site is novel for the parasite G6PD. The differences between parasite and human G6PD proteins could potentially be exploited for designing new chemotherapeutic agents.
寄生虫编码的葡萄糖-6-磷酸脱氢酶（PfG6PD）的结构可能为葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症患者对疟疾的相对抵抗力提供线索。我们克隆了Pfg6pd cDNA，其编码一个预测的含有856个氨基酸残基的多肽，计算分子量>94 kDa。预测的氨基酸序列与其他生物体的G6PD高度同源。Pfg6pd作为单拷贝或低拷贝数基因定位于14号染色体。寄生虫G6PD的N端异常大，且NADP结合位点与G6PD结合位点之间的距离是新发现的。寄生虫和人类G6PD蛋白之间的差异可能被用于设计新的化疗药物。
Zotero 插件