Roider J, Traccoli J, Michaud N, Flotte T, Anderson R, Birngruber R
Wellman Laboratories of Photomedicine, Boston.
Ophthalmologe. 1994 Jun;91(3):274-9.
The occlusion of arterioles and venules (30 microns in diameter) by means of repeated dye laser pulses (577 nm, 160 microseconds, 0.5 Hz) was studied in the hamster cheek pouch model. Microscopically visible changes during and after irradiation were recorded and monitored by a video system. The energy necessary per pulse for vessel occlusion with a single pulse (arterioles 5 J/cm2, venules 3.5 J/cm2) can be lowered more than 50% with 100 pulses (1.8 J/cm2, 1.2 J/cm2). Rupture of vessels, which regularly occurs with a single pulse, can be totally avoided with 100 repetitive laser pulses. Investigations of the temperature dependence of the occlusion rate of arterioles showed different interaction mechanisms with one pulse and 100 pulses (mechanical and thermal respectively). Light and electron microscopic investigations supported this concept of selective occlusion using repeated short laser pulses. Possible clinical applications are portwine stains of the eyelid, neovascularisation on the cornea and subretinal neovascularisation.
在仓鼠颊囊模型中，研究了通过重复染料激光脉冲（577纳米，160微秒，0.5赫兹）对直径为30微米的小动脉和小静脉的闭塞情况。照射期间和照射后的显微镜可见变化由视频系统记录和监测。单脉冲使血管闭塞所需的能量（小动脉5焦耳/平方厘米，小静脉3.5焦耳/平方厘米）在100次脉冲时（1.8焦耳/平方厘米，1.2焦耳/平方厘米）可降低超过50%。单脉冲时经常发生的血管破裂，在100次重复激光脉冲时可完全避免。对小动脉闭塞率的温度依赖性研究表明，单脉冲和100次脉冲时存在不同的相互作用机制（分别为机械和热机制）。光学和电子显微镜研究支持了使用重复短激光脉冲进行选择性闭塞的这一概念。可能的临床应用包括眼睑葡萄酒色斑、角膜新生血管和视网膜下新生血管。
