作为微小牛蜱中肠膜抗原佐剂的Quil A和免疫刺激复合物（ISCOMs）

Quil A and ISCOMs as adjuvants for midgut membrane antigens of Boophilus microplus.

作者信息

Jackson L A, Opdebeeck J P

机构信息

Department of Parasitology, University of Queensland, St. Lucia, Australia.

出版信息

Appl Parasitol. 1994 Jun;35(2):87-98.

PMID:8087157

Abstract

Quil A used with Boophilus microplus gut membrane antigen (GM) had a significant effect on antibody levels induced in sheep (P < 0.05) since GM alone did not induce a significant level of antibodies. Injection of a vaccine containing GM and Quil A, either subcutaneously or intramuscularly, induced similar levels of antibodies in sheep. However, Quil A injected subcutaneously induced acute inflammatory reaction. The amount of Quil A for use with GM was determined to be 1000 micrograms/ml. Immunostimulating complexes (ISCOMs) incorporating detergent-solubilized membrane midgut antigens (TX-GM) failed to induce an immune response in cattle without the addition of Quil A. The addition of Quil A to the ISCOMs containing TX-GM did not stimulate antibody responses greater than those stimulated by TX-GM plus Quil A, and protection in vaccinated cattle was 86% and 74%, respectively.

摘要

将Quil A与微小牛蜱肠膜抗原（GM）一起使用，对绵羊体内诱导产生的抗体水平有显著影响（P < 0.05），因为单独使用GM不会诱导产生显著水平的抗体。皮下或肌肉注射含有GM和Quil A的疫苗，在绵羊体内诱导产生的抗体水平相似。然而，皮下注射Quil A会引发急性炎症反应。确定与GM一起使用的Quil A用量为1000微克/毫升。在不添加Quil A的情况下，掺入经去污剂增溶的中肠膜抗原（TX-GM）的免疫刺激复合物（ISCOMs）未能在牛体内诱导免疫反应。在含有TX-GM的ISCOMs中添加Quil A，刺激产生的抗体反应并不比TX-GM加Quil A刺激产生的抗体反应更强，且接种疫苗的牛的保护率分别为86%和74%。