Opdebeeck J P, Wong J Y, Dobson C
Department of Parasitology, University of Queensland, St. Lucia, Australia.
Immunology. 1989 Jul;67(3):388-93.
Tick larvae (Boophilus microplus) were extracted, fractionated by gel filtration and ion-exchange chromatography, and the fractions assayed by enzyme-linked immunosorbent assay (ELISA) against sera from cattle immunized and protected by antigens from the mid-gut of adult ticks. Unfractionated, whole larval extracts and selected reactive components were used to vaccinate cattle; they decreased the number of ticks dropped after challenge, but the effect of treatment was not significant. Antigens purified from crude larval extracts by affinity chromatography, using immunoglobulin ligands from an immune steer vaccinated with tick mid-gut antigens, also failed to protect cattle. However, tick extracts from both larval and adult ticks, which were first separated into membrane-associated and soluble fractions and then purified by exposure to the affinity ligands, protected cattle greater than 80% against subsequent challenge with ticks.
提取蜱幼虫（微小牛蜱），通过凝胶过滤和离子交换色谱法进行分级分离，然后采用酶联免疫吸附测定（ELISA）法，用成年蜱中肠抗原免疫并保护的牛血清检测各分级分离物。未分级的全幼虫提取物和选定的反应性成分用于给牛接种疫苗；它们减少了攻击后掉落的蜱的数量，但治疗效果不显著。使用来自接种蜱中肠抗原的免疫公牛的免疫球蛋白配体，通过亲和色谱法从粗幼虫提取物中纯化的抗原，也未能保护牛。然而，首先分离为膜相关部分和可溶性部分，然后通过暴露于亲和配体进行纯化的幼虫和成年蜱的蜱提取物，可使牛对随后的蜱攻击产生大于80%的保护作用。
