Delgado J, Gómez E, Palma J L, González J, Monteseirin F J, Martínez A, Martínez J, Conde J
Servicio regional de Inmunología Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
Clin Exp Allergy. 1994 May;24(5):477-80. doi: 10.1111/j.1365-2222.1994.tb00937.x.
This paper highlights a clinical case of a patient suffering bronchial asthma and rhinoconjunctivitis due to sensitization to Tetranychus urticae (TU), commonly known as the red spider mite, which belongs to the Prostigmata sub-order of the Tetranychidae family, in relation to a work environment (a carnation nursery). Both prick and intradermal skin tests were positive, as well as specific bronchial challenge tests with TU extract. Specific IgE was demonstrated by RAST (Class 3). Unspecific bronchial provocation with methacholine was negative. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) immunoblotting revealed the presence of seven main IgE binding proteins, the more intense bands being those appearing at 21, 17 and 15 kDa. This indicates a case of immediated type hypersensitivity to Tetranychus urticae with a clear correlation to occupational environment.
本文着重介绍了一名因对二斑叶螨（Tetranychus urticae，俗称红蜘蛛螨）致敏而患有支气管哮喘和鼻结膜炎的临床病例，该螨属于叶螨科前气门亚目，与工作环境（康乃馨苗圃）有关。点刺试验和皮内试验均呈阳性，用二斑叶螨提取物进行的特异性支气管激发试验也呈阳性。通过放射性变应原吸附试验（RAST，3级）证实了特异性IgE的存在。用乙酰甲胆碱进行的非特异性支气管激发试验为阴性。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）免疫印迹显示存在七种主要的IgE结合蛋白，其中强度较高的条带出现在21、17和15 kDa处。这表明该病例为对二斑叶螨的速发型超敏反应，且与职业环境有明显关联。
