Springer G F, Yang H J
Vox Sang. 1978;35(4):255-64. doi: 10.1111/j.1423-0410.1978.tb02931.x.
Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with human antisera differed somewhat, while the specificity of mildly oxidized N-glycoprotein was completely destroyed as determined with all 8 human anti-N sera used, that of strongly oxidized N-active substance was completely inactivated towards one of the human antisera; the remainder showed 63--94% destruction, and 2 sera indicated no effect of oxidation. Similarly, while 10 of 14 human anti-M indicated complete inactivation of M-specific glycoproteins and glycopeptides after mild or strong oxidation, 2 showed partial inactivation and 2 human anti-M sera showed no inactivating effect of oxidation. The most relevant findings of quantitative carbohydrate analysis of periodate oxidized N- and M-specific substance were extensive transformation of N-acetylneuraminic acid (NAN) to its C8 and C1 analogues on mild oxidation and pronounced destruction of NAN and its analogues on strong oxidation; however, some intact NAN always remained. In all instances galactose (Gal) was destroyed to a much larger extent in N-derived than in M-derived glycoproteins and glycopeptides.
用蚕豆提取物检测时，对分离出的红细胞N和M糖蛋白及糖肽进行轻度和强烈的高碘酸盐氧化，会导致N特异性广泛至完全破坏；用8种抗N动物血清中的7种和13种抗M血清检测时，N活性和M活性也会被广泛至完全破坏。人抗血清的结果有所不同，在用所有8种人抗N血清检测时，轻度氧化的N糖蛋白的特异性完全被破坏，而强烈氧化的N活性物质对其中一种人抗血清完全失活；其余人抗血清显示63%-94%的破坏率，2种血清显示氧化无影响。同样，在14种人抗M血清中，10种显示轻度或强烈氧化后M特异性糖蛋白和糖肽完全失活，2种显示部分失活，2种人抗M血清显示氧化无失活作用。对高碘酸盐氧化的N和M特异性物质进行定量碳水化合物分析的最相关发现是，轻度氧化时N-乙酰神经氨酸（NAN）广泛转化为其C8和C1类似物，强烈氧化时NAN及其类似物明显被破坏；然而，总是会残留一些完整的NAN。在所有情况下，N衍生的糖蛋白和糖肽中半乳糖（Gal）的破坏程度比M衍生的要大得多。
