Kordowicz M, Lisowska E
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):127-32.
The polypeptide chains, containing only small amounts of hexosamines, were obtained from M and N blood groups glycoproteins by four cycles of periodate oxidation-Smith degradation, and were used for immunization of rabbits. The antisera obtained precipitated with the Smith-degraded glycoproteins, they reacted much weaker with the desialylated glycoproteins and they did not precipitate the untreated glycoproteins. In the quantitative microprecipitation assay the antisera precipitated better with the antigens of the same blood group specificity (M or N than those used for immunization. This blood group specificity was significantly increased by the cross-absorption of antisera.
通过高碘酸盐氧化-史密斯降解的四个循环，从M和N血型糖蛋白中获得了仅含有少量己糖胺的多肽链，并将其用于兔免疫。所获得的抗血清与经史密斯降解的糖蛋白发生沉淀反应，与去唾液酸糖蛋白的反应较弱，且不与未处理的糖蛋白发生沉淀反应。在定量微量沉淀试验中，抗血清与具有相同血型特异性（M或N）的抗原的沉淀效果比用于免疫的抗原更好。通过抗血清的交叉吸收，这种血型特异性显著增强。
