Zhao X J, Cihlar R L
Department of Microbiology and Immunology, Georgetown University, Washington, DC 20007.
Gene. 1994 Sep 15;147(1):119-24. doi: 10.1016/0378-1119(94)90050-7.
The gene (FAS1) encoding the beta-subunit of fatty-acid synthase (FAS) of Candida albicans has been isolated and analyzed. The gene was isolated on the basis of homology to the Saccharomyces cerevisiae FAS1 gene. Sequence analysis showed that the gene contained an intron-free open reading frame of 6111 bp encoding a protein of 2037 amino acids (aa) (227 916 Da). C. albicans FAS1 and its product exhibit a high degree of overall sequence relatedness to their counterparts in S. cerevisiae, with identities of 68 and 63% at the nucleotide (nt) and aa level, respectively. In addition, the beta-subunits of both organisms contain the catalytic domains in an identical sequential order. Northern blots demonstrated that the gene encodes a single mRNA of approx. 6.1 kb, and that changes in transcript levels are not a prerequisite of the yeast-to-hyphal transition. Southern blot analysis of C. albicans chromosomes separated by pulsed-field gel electrophoresis showed that FAS1 resides on chromosome 5.
白色念珠菌脂肪酸合酶（FAS）β亚基的编码基因（FAS1）已被分离和分析。该基因是基于与酿酒酵母FAS1基因的同源性而分离得到的。序列分析表明，该基因包含一个6111 bp的无内含子开放阅读框，编码一个由2037个氨基酸（aa）组成的蛋白质（227916 Da）。白色念珠菌FAS1及其产物与酿酒酵母中的对应物在整体序列上具有高度相关性，在核苷酸（nt）和氨基酸水平上的同一性分别为68%和63%。此外，两种生物体的β亚基都以相同的顺序包含催化结构域。Northern印迹显示该基因编码一种约6.1 kb的单一mRNA，并且转录水平的变化不是酵母向菌丝转变的先决条件。通过脉冲场凝胶电泳分离的白色念珠菌染色体的Southern印迹分析表明，FAS1位于5号染色体上。
