Saporito S M, Sypherd P S
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Gene. 1991 Sep 30;106(1):43-9. doi: 10.1016/0378-1119(91)90564-r.
Candida albicans is a dimorphic, opportunistic pathogen of humans, and calcium and calmodulin have been implicated in its morphogenic transition. The C. albicans calmodulin-encoding gene, CMD1, was isolated from cDNA and genomic phage lambda libraries using the Saccharomyces cerevisiae CMD gene as a hybridization probe. Southern-blot hybridization analysis of genomic DNA suggests the existence of only one type of calmodulin gene in C. albicans. Comparison of cDNA and genomic sequences identified a 222-bp intron located immediately after the Met start codon. The predicted amino acid sequence was 60% identical with yeast CMD and 70% identical with CMDs of filamentous fungi and vertebrates. We have localized the CMD1 gene to chromosome 3 using the contour-clamped homogeneous electric field gel electrophoresis. The CMD1 gene hybridized to a single 650-nucleotide transcript which was present in equivalent amounts in both the yeast and hyphal forms of the organism.
白色念珠菌是一种人类双态性机会致病菌，钙和钙调蛋白与其形态发生转变有关。利用酿酒酵母CMD基因作为杂交探针，从cDNA和基因组噬菌体λ文库中分离出白色念珠菌钙调蛋白编码基因CMD1。基因组DNA的Southern印迹杂交分析表明，白色念珠菌中仅存在一种类型的钙调蛋白基因。cDNA和基因组序列的比较确定了一个位于Met起始密码子之后的222 bp内含子。预测的氨基酸序列与酵母CMD的同源性为60%，与丝状真菌和脊椎动物的CMDs的同源性为70%。我们使用轮廓夹钳均匀电场凝胶电泳将CMD1基因定位到3号染色体上。CMD1基因与一个650个核苷酸的转录本杂交，该转录本在该生物体的酵母和菌丝形态中含量相等。
