Desai S S, Goud A P, Tendolkar A G, Rao S G
Chemotherapy & Stem Cell Biology Division, Tata Memorial Centre, Bombay, India.
Indian J Exp Biol. 1994 Apr;32(4):225-8.
Stem cell adhesion to bone-marrow derived stroma, plays a crucial role in haemopoiesis. However, there is very little information as to the nature of the adhesion molecule. In this paper we have shown that human bone-marrow derived stroma can be established in tissue culture. This stroma is able to adhere human bone-marrow mononuclear cells including the multipotent stem cell, viz. CFU-GEMM. Their adherence increases when the stroma is treated with lymphokines in the form of PHA-treated leucocyte conditioned medium (PHA-LCM). Triton X-100 extracts of the untreated and PHA-LCM treated stroma were analysed on single dimension PAGE. It was observed that PHA-LCM treated stromal extracts showed two extra bands and an increase in the density of a band of approximately 14 kDa. Whether these changes have anything to do with the increased adhesion of stem cell is not yet known.
干细胞与骨髓来源的基质的黏附在造血过程中起着关键作用。然而，关于黏附分子的性质却知之甚少。在本文中，我们已经表明可以在组织培养中建立人骨髓来源的基质。这种基质能够黏附包括多能干细胞即CFU - GEMM在内的人骨髓单核细胞。当用PHA处理的白细胞条件培养基（PHA - LCM）形式的淋巴因子处理基质时，它们的黏附性会增加。对未处理和PHA - LCM处理的基质的Triton X - 100提取物进行一维聚丙烯酰胺凝胶电泳分析。观察到PHA - LCM处理的基质提取物显示出两条额外的条带以及一条约14 kDa条带的密度增加。这些变化是否与干细胞黏附性增加有关尚不清楚。
