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[利用细胞回收器结合膜滤器分离泌尿肿瘤细胞。一种自体输血的新技术?]

[Separation of urological tumor cells by means of a cell saver combined with a membrane filter. A new technique for autotransfusion?].

作者信息

Wiesel M, Carl S, Güdemann C, Brua E, Hoever K H, Staehler G

机构信息

Service d'Urologie, Klinikum der Univ. Heidelberg, Allemagne.

出版信息

J Urol (Paris). 1994;100(1):29-32.

PMID:8089527
Abstract

Three long duration cancer cell lines were marked with radioactive methionine S and mixed with a defined quantity of concentrated red cells. The mixture was separated according to a standardized method with cell saver and two membrane filters. The marked cancer cells were counted with a liquid scintillation cell counter. After separation, only 0.027% of the initial radioactivity was retrieved. If this result can be confirmed in a clinical setting, this technique would be highly promising for intraoperative automatic autotransfusions, especially in the field of tumour surgery. Despite the quality of conserved blood, heterotransfusion still involves considerable risk for the receiver.

摘要

三种长期存活的癌细胞系用放射性甲硫氨酸 S 进行标记,并与一定量的浓缩红细胞混合。采用标准化方法,使用细胞保存器和两个膜过滤器对混合物进行分离。用液体闪烁细胞计数器对标记的癌细胞进行计数。分离后,仅回收了初始放射性的0.027%。如果这一结果能在临床环境中得到证实,那么这项技术在术中自动自体输血方面将极具前景,尤其是在肿瘤手术领域。尽管保存血液的质量有所提高,但异体输血对接受者仍存在相当大的风险。

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