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[使用膜滤器从细胞保存血液中分离泌尿系统肿瘤细胞。自体输血的一种新方法?]

[Separation of urologic tumors cells from Cell Saver blood using a membrane filter. A new method in autotransfusion?].

作者信息

Wiesel M, Güdemann C, Staehler G, Bierhaus A, Martin E, Hoever K H

机构信息

Abteilung für Urologie, Klinikum der Universität, Heidelberg.

出版信息

Urologe A. 1992 May;31(3):182-5.

PMID:1615592
Abstract

Tumor cells of three urological longterm cell lines have been labelled with 35[S]-Methionin and added to red cell concentrates. Red cell concentrates rich in tumor cells were passed through a cell saver and two special membranfilters under standard conditions. The 35[S]-Methionin labelled tumor cells were detected by liquid scintillation counting. On an average, only 0.027% of the radioactivity was left after passing through the cell saver and the membran filters in the 12 experiments. If investigations in clinical use of cell-saver and membran filter confirm these results, there will be significant consequences in urological tumor surgery by the possibility of transfusing the salvaged autologous blood.

摘要

三种泌尿系统长期细胞系的肿瘤细胞已用35[S]-甲硫氨酸标记,并添加到红细胞浓缩物中。富含肿瘤细胞的红细胞浓缩物在标准条件下通过细胞回收器和两个特殊的膜过滤器。通过液体闪烁计数检测35[S]-甲硫氨酸标记的肿瘤细胞。在12次实验中,平均而言,经过细胞回收器和膜过滤器后,仅剩下0.027%的放射性。如果关于细胞回收器和膜过滤器临床应用的研究证实了这些结果,那么在泌尿系统肿瘤手术中,通过输注回收的自体血的可能性将会产生重大影响。

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