Zhou X F, Williams R, Zecevic M, Rush R A
Centre for Neuroscience, Flinders University of South Australia, Adelaide.
J Neurocytol. 1994 May;23(5):271-7. doi: 10.1007/BF01188496.
Previous studies from this laboratory have used antisera to aldehyde-conjugated ovalbumin to localize ovalbumin-like immunoreactivity within a subpopulation of sensory neurons. We have now combined retrograde tracing and immunohistochemical procedures to identify the tissues innervated by sensory neurons which are either immunoreactive or non-immunoreactive for ovalbumin. The fluorescent tracer Di-I was administered to feather follicles, flexor ulnar muscle, subdermis, expansor secundariorum, heart and liver and identified seven days later within corresponding dorsal root ganglia. Most neurons innervating the follicles had large cell somata, and fewer than 3% were immunoreactive for ovalbumin. In contrast, most sensory neurons projecting to subdermis, muscle and expansor secundariorum muscle were of a medium diameter. Approximately 25% of those neurons projecting to the expansor secundariorum, and 60% projecting to the subdermis and muscle, were immunoreactive for ovalbumin. Sensory neurons innervating heart and liver were the smallest, and only 8% were immunoreactive for ovalbumin. The study indicates that sensory neurons innervating different organs have somata with significantly different sizes, suggesting a functional specificity. Moreover, neurons demonstrating either the ovalbumin-IR positive or negative phenotypes show distinct peripheral projections, suggesting that this phenotype may be at least partially controlled by retrograde signals derived from the cells they innervate.
该实验室之前的研究使用了针对醛结合卵清蛋白的抗血清，以在感觉神经元亚群中定位类似卵清蛋白的免疫反应性。我们现在将逆行追踪和免疫组织化学方法相结合，以识别由对卵清蛋白呈免疫反应性或非免疫反应性的感觉神经元所支配的组织。将荧光示踪剂Di-I注射到毛囊、尺侧屈肌、皮下组织、次级扩张肌、心脏和肝脏中，并在七天后在相应的背根神经节中识别出来。支配毛囊的大多数神经元具有大的细胞体，对卵清蛋白呈免疫反应性的不到3%。相比之下，投射到皮下组织、肌肉和次级扩张肌的大多数感觉神经元直径中等。投射到次级扩张肌的神经元中约25%，投射到皮下组织和肌肉的神经元中60%，对卵清蛋白呈免疫反应性。支配心脏和肝脏的感觉神经元最小，只有8%对卵清蛋白呈免疫反应性。该研究表明，支配不同器官的感觉神经元具有大小明显不同的细胞体，这表明存在功能特异性。此外，表现出卵清蛋白免疫反应阳性或阴性表型的神经元显示出不同的外周投射，这表明这种表型可能至少部分受其支配细胞衍生的逆行信号控制。
