视网膜色素光谱调谐中的协同作用：细菌视紫红质视蛋白移位的完整核算。
Synergy in the spectral tuning of retinal pigments: complete accounting of the opsin shift in bacteriorhodopsin.
作者信息
Hu J, Griffin R G, Herzfeld J
机构信息
Department of Chemistry, Brandeis University, Waltham, MA 02254-9110.
出版信息
Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8880-4. doi: 10.1073/pnas.91.19.8880.
Abstract
UV-visible and solid-state NMR studies of a series of 6-s-trans protonated Schiff bases of retinal with aniline show that the bathochromic shift induced by weakening the imine counterion is significantly greater in the 6-s-trans conformation than in the 6-s-cis conformation. Based on the observed magnitude of this coupling between the electronic effects of 6-s isomerization and imine counterion strength in the model compounds, the large opsin shift and unusual chemical shifts in light-adapted bacteriorhodopsin can be fully explained. These phenomena therefore do not require a negative point charge or polarizability effects in the chromophore binding pocket. The results are consistent with an effective center-to-center distance between the Schiff base and its counterion of about 4 A in light-adapted bacteriorhodopsin.
摘要
对一系列视网膜与苯胺形成的6-s-反式质子化席夫碱进行的紫外可见光谱和固态核磁共振研究表明，在6-s-反式构象中，因亚胺抗衡离子减弱而引起的红移比在6-s-顺式构象中显著更大。基于在模型化合物中观察到的6-s异构化的电子效应与亚胺抗衡离子强度之间这种耦合的大小，光适应型细菌视紫红质中较大的视蛋白位移和异常的化学位移可以得到充分解释。因此，这些现象不需要发色团结合口袋中存在负点电荷或极化率效应。结果与光适应型细菌视紫红质中席夫碱与其抗衡离子之间约4埃的有效中心距一致。
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本文引用的文献
1
Studies on rhodopsin. VIII. Retinylidenemethylamine, an indicator yellow analogue.视紫红质的研究。VIII. 视黄亚基甲基胺，一种类似指示剂黄的物质。
Biochem J. 1955 Jan;59(1):122-8. doi: 10.1042/bj0590122.
2
The mechanism of bleaching rhodopsin.视紫红质的漂白机制。
Ann N Y Acad Sci. 1959 Nov 12;74(2):266-80. doi: 10.1111/j.1749-6632.1958.tb39550.x.
3
Solid-state nitrogen-15 nuclear magnetic resonance study of the Schiff base in bacteriorhodopsin.细菌视紫红质中席夫碱的固态氮-15核磁共振研究。
Biochemistry. 1983 Jan 4;22(1):1-4. doi: 10.1021/bi00270a600.
4
Effect of solvent polarizability on the absorption spectrum of all-trans-retinylpyrrolidiniminium perchlorate.
J Am Chem Soc. 1969 Apr 9;91(8):2141-3. doi: 10.1021/ja01036a066.
5
Anion-induced wavelength regulation of absorption maxima of Schiff bases of retinal.阴离子诱导的视网膜席夫碱吸收最大值的波长调节。
Biochemistry. 1972 Feb 29;11(5):848-55. doi: 10.1021/bi00755a026.
6
Solid-state 13C NMR detection of a perturbed 6-s-trans chromophore in bacteriorhodopsin.细菌视紫红质中受扰的6-s-反式发色团的固态13C核磁共振检测
Biochemistry. 1985 Nov 19;24(24):6955-62. doi: 10.1021/bi00345a031.
7
Nuclear magnetic resonance study of the Schiff base in bacteriorhodopsin: counterion effects on the 15N shift anisotropy.细菌视紫红质中席夫碱的核磁共振研究：抗衡离子对15N位移各向异性的影响
Biochemistry. 1989 Apr 18;28(8):3346-53. doi: 10.1021/bi00434a033.
8
Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.基于高分辨率电子冷冻显微镜的细菌视紫红质结构模型。
J Mol Biol. 1990 Jun 20;213(4):899-929. doi: 10.1016/S0022-2836(05)80271-2.
9
Solid-state 13C and 15N NMR study of the low pH forms of bacteriorhodopsin.细菌视紫红质低pH形式的固态¹³C和¹⁵N核磁共振研究。
Biochemistry. 1990 Jul 24;29(29):6873-83. doi: 10.1021/bi00481a017.
10
Retinal has a highly dipolar vertically excited singlet state: implications for vision.视黄醛具有高度偶极的垂直激发单重态：对视觉的影响。
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2169-73. doi: 10.1073/pnas.73.7.2169.
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