Levée G, Glaziou P, Gicquel B, Chanteau S
Institut Territorial de Recherches Médicales Louis Malardé, Papeete, Tahiti.
Res Microbiol. 1994 Jan;145(1):5-8. doi: 10.1016/0923-2508(94)90061-2.
We have analysed the clearance of Mycobacterium tuberculosis in sputum specimens from pulmonary tuberculosis patients undergoing 6-month chemotherapy, using the polymerase chain reaction (PCR) and standard microbiological methods. In a group of 19 patients, 11 (58%) were smear- or culture-positive and 13 (74%) were PCR-positive before treatment. Of the 16 patients followed from 2 months after the start of treatment and thereafter, all became smear-negative and culture-negative, whereas, with PCR, 4 (27%), 2 (13%) and 1 (7%) of these patients remained positive after 2, 3 and 6 months, respectively. These results suggest the possible usefulness of PCR in monitoring the efficacy of treatment when bacteriological tests are negative, so as to identify patients with a high risk of relapse.
我们运用聚合酶链反应（PCR）和标准微生物学方法，分析了接受6个月化疗的肺结核患者痰液标本中结核分枝杆菌的清除情况。在一组19例患者中，11例（58%）在治疗前痰涂片或培养呈阳性，13例（74%）PCR检测呈阳性。在开始治疗2个月及之后随访的16例患者中，所有患者痰涂片和培养均转为阴性，而采用PCR检测时，这些患者在2个月、3个月和6个月后分别有4例（27%）、2例（13%）和1例（7%）仍呈阳性。这些结果表明，当细菌学检测为阴性时，PCR在监测治疗效果方面可能有用，以便识别复发风险高的患者。
