Bouvet O M, Lenormand P, Carlier J P, Grimont P A
Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, Paris.
Res Microbiol. 1994 Feb;145(2):129-39. doi: 10.1016/0923-2508(94)90006-x.
The anaerobic glycerol pathway was studied in seven enterobacterial species selected as representative of different behaviours in terms of anaerobic glycerol dissimilation. The presence of oxidative and reductive pathways of the dha regulon in Klebsiella pneumoniae enabled the cells to grow fermentatively on glycerol. The first two enzymes of the dha regulon (glycerol dehydrogenase type I and dihydroxyacetone kinase) represent the oxidative branch, while the latter two (glycerol dehydratase and 1,3-propanediol dehydrogenase) represent the reductive branch of glycerol fermentation. The slower utilization of glycerol by K. oxytoca was attributed to low production of 1,3-propanediol. K. oxytoca lacked glycerol dehydratase and demonstrated low 1,3-propanediol dehydrogenase activity. K. planticola and K. ozaenae differed from K. pneumoniae and K. oxytoca in lacking the ability to grow on glycerol. K. planticola lacked both enzymes of the reductive branch of glycerol fermentation, and K. ozaenae possessed glycerol dehydrogenase only. K. rhinoscleromatis and Hafnia alvei, like Escherichia coli, did not possess a dha regulon. The glycerol dehydrogenase type II of H. alvei was distinct from that of E. coli. The phenotypic diversity of anaerobic glycerol dissimilation may have taxonomic applications.
在七种肠杆菌中研究了厌氧甘油途径，这些肠杆菌被选为在厌氧甘油异化方面具有不同行为的代表。肺炎克雷伯菌中dha操纵子的氧化和还原途径的存在使细胞能够在甘油上进行发酵生长。dha操纵子的前两种酶（I型甘油脱氢酶和二羟基丙酮激酶）代表氧化分支，而后两种酶（甘油脱水酶和1,3 - 丙二醇脱氢酶）代表甘油发酵的还原分支。产酸克雷伯菌对甘油的利用较慢归因于1,3 - 丙二醇产量低。产酸克雷伯菌缺乏甘油脱水酶，并且1,3 - 丙二醇脱氢酶活性较低。植生克雷伯菌和臭鼻克雷伯菌与肺炎克雷伯菌和产酸克雷伯菌不同，它们缺乏在甘油上生长的能力。植生克雷伯菌缺乏甘油发酵还原分支的两种酶，而臭鼻克雷伯菌仅拥有甘油脱氢酶。鼻硬结克雷伯菌和蜂房哈夫尼亚菌与大肠杆菌一样，不具有dha操纵子。蜂房哈夫尼亚菌的II型甘油脱氢酶与大肠杆菌的不同。厌氧甘油异化的表型多样性可能具有分类学应用价值。
