Bouvet O M, Lenormand P, Ageron E, Grimont P A
Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, Paris.
Res Microbiol. 1995 May;146(4):279-90. doi: 10.1016/0923-2508(96)81051-5.
A total of 1,123 strains representing 128 taxa in the Enterobacteriaceae (named species or subspecies and genomic species) were screened for the presence of glycerol dehydrogenases and 1,3-propanediol dehydrogenase. Only eight taxa, Citrobacter freundii sensu stricto, C. youngae, C. braakii, C. werkmanii, Citrobacter genomospecies 10 and 11, Enterobacter gergoviae and Klebsiella pneumoniae subsp. pneumoniae could grow fermentatively on glycerol and possessed both glycerol dehydrogenase type I (induced by glycerol and dihydroxyacetone) and 1,3-propanediol dehydrogenase which are typical enzymes of the anaerobic glycerol dissimilation pathway. Six other species, C. koseri, E. aerogenes, E. intermedium, K. oxytoca, K. planticola and K. terrigena could not grow fermentatively on glycerol and possessed a glycerol dehydrogenase type I but no 1,3-propanediol dehydrogenase. Other glycerol dehydrogenases types were found: type II (induced by glycerol and hydroxyacetone), type III (induced by glycerol only) and type IV (induced by hydroxyacetone only). They were widely distributed among the Enterobacteriaceae. Classification and identification may take advantage of tests exploring the dissimilation of glycerol.
对总共1123株代表肠杆菌科128个分类单元（命名种或亚种以及基因组种）的菌株进行了甘油脱氢酶和1,3 - 丙二醇脱氢酶的筛选。只有8个分类单元，即弗氏柠檬酸杆菌狭义种、杨氏柠檬酸杆菌、布氏柠檬酸杆菌、韦氏柠檬酸杆菌、柠檬酸杆菌基因组种10和11、杰氏肠杆菌和肺炎克雷伯菌肺炎亚种，能够在甘油上进行发酵生长，并且同时拥有I型甘油脱氢酶（由甘油和二羟基丙酮诱导）和1,3 - 丙二醇脱氢酶，这两种酶是厌氧甘油异化途径的典型酶。其他6个种，即科氏柠檬酸杆菌、产气肠杆菌、中间肠杆菌、产酸克雷伯菌、栖植物克雷伯菌和土生克雷伯菌，不能在甘油上进行发酵生长，它们拥有I型甘油脱氢酶，但没有1,3 - 丙二醇脱氢酶。还发现了其他类型的甘油脱氢酶：II型（由甘油和羟基丙酮诱导）、III型（仅由甘油诱导）和IV型（仅由羟基丙酮诱导）。它们在肠杆菌科中广泛分布。分类和鉴定可以利用探索甘油异化作用的试验。
