Kitajima S, Ogawa N, Ariyoshi T
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
Res Commun Chem Pathol Pharmacol. 1994 May;84(2):203-22.
Oral administration of crude extracts of Trionyx carapax into rats was found to protect the liver from its CCl4-induced injury as judged by morphological and biochemical observations. The active principle was purified from crude extracts of Trionyx carapax by Sephadex G-75, Sephadex G-25, DEAE-cellulose and silica gel column chromatographies. The results of elemental analysis, infrared and mass spectroscopy demonstrated the purified component to be identical to uracil. Upon administration, authentic uracil exhibited the protective effects on CCl4-induced hepatic injury, a pattern identical to or very similar to that observed with crude extracts of Trionyx carapax. Thus it is concluded that uracil is the active component responsible for protection from CCl4-induced hepatic injury.
通过形态学和生化观察发现，给大鼠口服鳖甲粗提物可保护肝脏免受四氯化碳诱导的损伤。通过葡聚糖凝胶G - 75、葡聚糖凝胶G - 25、二乙氨基乙基纤维素和硅胶柱色谱法从鳖甲粗提物中纯化出活性成分。元素分析、红外光谱和质谱结果表明，纯化后的成分与尿嘧啶相同。给予尿嘧啶后，其对四氯化碳诱导的肝损伤具有保护作用，这一模式与鳖甲粗提物所观察到的相同或非常相似。因此得出结论，尿嘧啶是负责保护肝脏免受四氯化碳诱导肝损伤的活性成分。
