Ohta Y, Sasaki E, Nishida K, Kongo M, Hayashi T, Nagata M, Ishiguro I
Department of Biochemistry, School of Medicine, Fujita Health University, Aichi, Japan.
J Ethnopharmacol. 1998 May;61(1):75-80. doi: 10.1016/s0378-8741(98)00018-x.
The inhibitory effect of Oren-gedoku-to (Huanglian-Jie-Du-Tang) extract (TJ-15) on hepatic triglyceride (TG) accumulation with the progression of acute liver injury was examined in rats intoxicated with carbon tetrachloride (CCl4). TJ-15 at a dose of 100, 250 or 500 mg/kg body weight (BW) was orally administered to male Wistar rats aged 7 weeks, 6 h after the intraperitoneal injection of CCl4 (1.0 ml/kg BW) at which time apparent liver injury and hepatic TG accumulation occurred. TJ-15 significantly prevented not only the progression of liver injury but also inhibited hepatic TG accumulation with the progression of the injury in a dose-dependent manner when these effects were examined 24 h after CCl4 injection. In CCl4-untreated rats with oral administration of TJ-15 at a dose of 100, 250 or 500 mg/kg BW, liver and serum TG concentrations decreased depending on the dose of the herbal medicine. These results indicate that in rats intoxicated once with CCl4, orally administered TJ-15 can inhibit hepatic TG accumulation with the progression of acute liver injury by its decreasing action on serum and liver TG levels, leading to a prevention of the progression of the liver injury.
在四氯化碳（CCl4）中毒的大鼠中，研究了黄连解毒汤提取物（TJ - 15）对急性肝损伤进展过程中肝脏甘油三酯（TG）蓄积的抑制作用。将7周龄雄性Wistar大鼠腹腔注射CCl4（1.0 ml/kg体重）6小时后，此时出现明显肝损伤和肝脏TG蓄积，分别口服给予剂量为100、250或500 mg/kg体重的TJ - 15。在CCl4注射24小时后检查这些作用时，TJ - 15不仅显著阻止了肝损伤的进展，还以剂量依赖的方式抑制了随着损伤进展而出现的肝脏TG蓄积。在未注射CCl4但口服给予剂量为100、250或500 mg/kg体重TJ - 15的大鼠中，肝脏和血清TG浓度根据草药剂量而降低。这些结果表明，在一次性CCl4中毒的大鼠中，口服TJ - 15可通过降低血清和肝脏TG水平，抑制急性肝损伤进展过程中的肝脏TG蓄积，从而预防肝损伤的进展。
