Fiore J R, Björndal A, Peipke K A, Di Stefano M, Angarano G, Pastore G, Gaines H, Fenyö E M, Albert J
Department of Microbiology and Tumorbiology, Karolinska Institute, Stockholm, Sweden.
Virology. 1994 Oct;204(1):297-303. doi: 10.1006/viro.1994.1534.
The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) or intermediate (n = 1), while 14 isolates (67%) did not replicate in cell lines and were classified as slow/low. All isolates replicated in primary monocyte-derived macrophage cultures. Studies on 10 pairs consisting of index case and seroconverting sexual partner showed that the biological phenotype of HIV-1 was unchanged in nine transmissions, including 5 pairs where a rapid/high virus was transmitted. Most individuals (5 of 6) infected by a rapid/high virus retained this phenotype after seroconversion. These individuals also had significantly lower CD4 lymphocyte counts 1-3 years after infection. Thus, in contrast to what has been suggested by others, we found that HIV-1 variants with rapid/high phenotype were usually not suppressed, neither during sexual transmission nor after seroconversion in the new host. These findings have important implications for the understanding of HIV-1 transmission and pathogenesis as well as for vaccine design.
在原代淋巴细胞、单核细胞衍生的巨噬细胞以及三种T细胞系（MT - 2、HUT78和C8166）中，确定了21名原发性HIV - 1感染者的HIV - 1分离株的生物学表型。7株分离株（33%）在至少一种细胞系中复制，被分类为快速/高复制型（n = 6）或中间型（n = 1），而14株分离株（67%）在细胞系中不复制，被分类为缓慢/低复制型。所有分离株在原代单核细胞衍生的巨噬细胞培养物中均能复制。对由索引病例和血清转化的性伴侣组成的10对样本进行的研究表明，在9次传播中HIV - 1的生物学表型未发生变化，其中包括5对传播快速/高复制型病毒的样本。大多数（6例中的5例）感染快速/高复制型病毒的个体在血清转化后仍保留该表型。这些个体在感染后1至3年的CD4淋巴细胞计数也显著较低。因此，与其他人的观点相反，我们发现具有快速/高表型的HIV - 1变体在性传播期间以及在新宿主血清转化后通常不会受到抑制。这些发现对理解HIV - 1传播和发病机制以及疫苗设计具有重要意义。
