Scarlatti G, Hodara V, Rossi P, Muggiasca L, Bucceri A, Albert J, Fenyö E M
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Virology. 1993 Dec;197(2):624-9. doi: 10.1006/viro.1993.1637.
The aim of this study was to investigate if the risk of mother-to-child transmission of human immunodeficiency virus type 1 (HIV-1) is influenced by the biological phenotype of the mother's virus. Virus isolates from 30 HIV-1 infected mothers and 12 infected children born to these mothers were analyzed for replication on several cell lines (Jurkat-tat, Jurkat, CEM, U937 clone 2, and MT-2). We show that mothers who harbor virus able to replicate in cell lines (rapid/high virus) have a significantly higher risk to infect their children than mothers with slow/low virus (P = 0.017). Children born to mothers with rapid/high viruses can be infected by slow/low as well as rapid/high viruses, while mothers with slow/low virus appear to transmit slow/low virus in every case. Our study shows that the biological phenotype of the mother's virus may serve as a complementary marker to CD4+ lymphocyte counts and p24 antigenemia in predicting the risk of transmission of HIV-1 to the child.
本研究的目的是调查1型人类免疫缺陷病毒（HIV-1）母婴传播风险是否受母亲病毒生物学表型的影响。对来自30名HIV-1感染母亲及其所生的12名感染儿童的病毒分离株进行分析，检测其在几种细胞系（Jurkat-tat、Jurkat、CEM、U937克隆2和MT-2）中的复制情况。我们发现，携带能够在细胞系中复制的病毒（快速/高病毒载量）的母亲感染其子女的风险显著高于携带慢速/低病毒载量的母亲（P = 0.017）。携带快速/高病毒载量的母亲所生的儿童可能会被慢速/低病毒载量以及快速/高病毒载量的病毒感染，而携带慢速/低病毒载量的母亲似乎在每种情况下都传播慢速/低病毒载量的病毒。我们的研究表明，母亲病毒的生物学表型在预测HIV-1传播给儿童的风险方面，可能作为CD4 +淋巴细胞计数和p24抗原血症的补充标志物。
