Martin R, Börner H, Martin H, Rotzsch W
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universität Leipzig.
Z Gerontol. 1993 Jul-Aug;26(4):238-42.
The nuclei binding of activated glucocorticoid receptor complexes (GRC) was significantly diminished in old, unstressed rats compared with young ones. In animals killed at different times after physiological stress there were no significant age-dependent differences in nuclei binding of GRC, which recurred in the cytoplasm after 60, 120 and 180 min, resp. Addition of Ca2+ and ATP, resp., to the incubation system increased the nuclei bound part of GRC, especially in young animals. ATP-stimulated translocation promoter (ASTP), isolated from rat liver cytosol, raised the nuclei binding of GRC in both age groups, whereby the increase was only significant in young animals. In cytosol of old rats ASTP were found in a lower concentration; moreover, the reaction between ASTP, GRC, and the template seems to be impaired.
与年轻大鼠相比，未受应激的老年大鼠中活化糖皮质激素受体复合物（GRC）的核结合显著减少。在生理应激后不同时间处死的动物中，GRC的核结合没有明显的年龄依赖性差异，分别在60、120和180分钟后在细胞质中再次出现。向孵育系统中分别添加Ca2+和ATP可增加GRC的核结合部分，尤其是在年轻动物中。从大鼠肝细胞质溶胶中分离出的ATP刺激转运启动子（ASTP）提高了两个年龄组中GRC的核结合，其中仅在年轻动物中增加显著。在老年大鼠的细胞质溶胶中发现ASTP浓度较低；此外，ASTP、GRC和模板之间的反应似乎受到损害。
