Yoshimura M, Ochi Y, Hachiya T, Miyazaki T
Endocrinol Jpn. 1975 Jun;22(3):199-205. doi: 10.1507/endocrj1954.22.199.
A new method for the estimation of the maximal T4-binding capacity of TBG (max. TBG) in serum (dextran-coated charcoal-triosorb method) was devised. Thyroxine (T4) concentration in test sera were determined by the Tetrasorb method and then T3 resin sponge uptake (T3 RSU) was determined after extraction of 70% of the endogenous T4 with dextran-coated charcoal. The binding capacity of unsaturated TBG in treated sera was estimated from the reciprocal of T3 RSU. Max. TBG was calculated from the arithmetic sum of the unsaturated binding capacity of TBG and the serum T4 concentration multiplied by 0.3. The normal range for max. TBG was 20.1 +/- 2.6 (mean +/- SD) mug T4/100 ml (14 approximately 24) and it was 19.2 +/- 3.5 mug T4/100 ml in hyperthyroidism which was lower compared with hypothyroidism (21.1 +/- 2.6 mug T4/100 ml). Max. TBG in 8 hyperthyroid patients did not change significantly after treatment in half of the case, although in the other half it was slightly increased. Increased values of max. TBG were observed in pregnancy (32.9 +/- 4.4) and in a hyperthyroid state associated with pregnancy (31.4 +/- 4.7). Decreases were observed in TBG deficiency (less than 5.6 mug T4/100 ml). These procedures can be applied for the determination of both the unsaturated binding capacity of TBG and that of max. TBG.
设计了一种估算血清中甲状腺素结合球蛋白（TBG）最大T4结合能力（最大TBG）的新方法（葡聚糖包被活性炭-三碘甲状腺原氨酸吸附法）。用四碘甲状腺原氨酸吸附法测定检测血清中的甲状腺素（T4）浓度，然后用葡聚糖包被活性炭提取70%的内源性T4后，测定T3树脂海绵摄取率（T3 RSU）。根据T3 RSU的倒数估算处理后血清中未饱和TBG的结合能力。最大TBG通过将TBG的未饱和结合能力与血清T4浓度乘以0.3的算术和来计算。最大TBG的正常范围为20.1±2.6（均值±标准差）μg T4/100 ml（14～24），甲状腺功能亢进症患者为19.2±3.5μg T4/100 ml，低于甲状腺功能减退症患者（21.1±2.6μg T4/100 ml）。8例甲状腺功能亢进症患者中，半数患者治疗后最大TBG无明显变化，另一半则略有升高。妊娠（32.9±4.4）和妊娠合并甲状腺功能亢进状态（31.4±4.7）时观察到最大TBG值升高。TBG缺乏症患者（低于5.6μg T4/100 ml）出现降低。这些方法可用于测定TBG的未饱和结合能力和最大TBG的未饱和结合能力。
