玉米中不依赖辅因子的磷酸甘油酸变位酶基因的分离与鉴定

Isolation and characterization of cofactor-independent phosphoglycerate mutase gene from maize.

作者信息

Pérez de la Ossa P, Graña X, Ruiz-Lozano P, Climent F

机构信息

Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.

出版信息

Biochem Biophys Res Commun. 1994 Sep 15;203(2):1204-9. doi: 10.1006/bbrc.1994.2310.

DOI:10.1006/bbrc.1994.2310

Abstract

The isolation and characterization of cofactor-independent phosphoglycerate mutase gene from maize (Zea mays) is here reported. This gene sequence constitutes the first described from this enzyme. The gene spans 5.4 kilo base pairs and has nine exons in the translated region. Canonical TATA and CCAAT and Sp1-like boxes are present upstream of the gene. Comparison with cofactor-dependent phosphoglycerate mutase genes revealed no similarity between both groups of enzymes.

摘要

本文报道了从玉米（Zea mays）中分离和鉴定不依赖辅因子的磷酸甘油酸变位酶基因。该基因序列是首次报道的这种酶的序列。该基因跨度为5.4千碱基对，在翻译区域有9个外显子。在基因上游存在典型的TATA、CCAAT和类Sp1框。与依赖辅因子的磷酸甘油酸变位酶基因比较发现，这两组酶之间没有相似性。

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