多发性硬化症患者血清中髓鞘的生物合成及神经毒性因子

Biosynthesis of myelin and neurotoxic factors in the serum of multiple sclerosis patients.

作者信息

Davison A N, Sabri M I

出版信息

Adv Exp Med Biol. 1978;100:19-25. doi: 10.1007/978-1-4684-2514-7_2.

Abstract

The in vitro synthesis of myelin proteins has been studied by measuring the incorporation of [3H] lysine in developing rat brain slices. This incorporation system has been used to assay potentially gliotoxic and myelinolytic agents. A reduced incorporation of the labelled amino acid into myelin proteins occurs in the presence of anti-myelin anti-serum and anti-basic protein anti-serum. Diphtheria toxin has been found to inhibit the synthesis of myelin basic and proteolipid protein in the white matter slices of developing rats. Recent experiments with serum samples from multiple sclerosis patients in exacerbation suggest the presence of a factor which interferes with the synthesis of myelin in white matter slices.

摘要

通过测量[3H]赖氨酸在发育中大鼠脑切片中的掺入情况，对髓鞘蛋白的体外合成进行了研究。该掺入系统已被用于检测潜在的神经胶质毒性和髓鞘溶解剂。在抗髓鞘抗血清和抗碱性蛋白抗血清存在的情况下，标记氨基酸掺入髓鞘蛋白的量减少。已发现白喉毒素可抑制发育中大鼠白质切片中髓鞘碱性蛋白和蛋白脂蛋白的合成。最近对病情加重的多发性硬化症患者血清样本进行的实验表明，存在一种干扰白质切片中髓鞘合成的因子。