Schwaeble W, Kerlin M, Meyer zum Büschenfelde K H, Dippold W
Department of Internal Medicine, Johannes Gutenberg-Universität, Mainz, Germany.
Int J Cancer. 1993 Jan 21;53(2):328-33. doi: 10.1002/ijc.2910530226.
We examined the expression of intercellular--adhesion molecule-I (ICAM-I, CD54) in 6 surgically removed pancreatic tumors and 8 pancreatic tumor cell lines. Immunohistochemistry revealed a varying percentage of ICAM-I-positive pancreas tumor cells, while normal pancreatic tissue (except for slight reactivity of endothelial cells) was not stained. The presence of the ICAM-I molecule on the cell surface and the expression of ICAM-I mRNA were investigated for 8 different pancreatic tumor cell lines. Three of these (Capan-I, Capan-2, QGP-I) expressed ICAM-I constitutively. In 4 of the ICAM-I-negative pancreas cancer cell lines, it was possible to induce a remarkable expression of ICAM-I by incubating the cells in the presence of inflammatory cytokines, whereas one cell line, 818-4, remained ICAM-I-negative. The responsiveness to either IFN-gamma, TNF-alpha, or IL-I beta treatment was shown to vary from cell line to cell line, indicating complex mechanisms that regulate the expression of ICAM-I at both, the transcriptional and the post-transcriptional level. Interestingly, ICAM-I is shed by pancreatic tumor cells, since soluble sICAM-I was detected in the cell-culture supernatants. In comparison with normal sera, the mean level of sICAM-I in sera of patients with pancreas carcinoma is elevated 2-fold.
我们检测了6例手术切除的胰腺肿瘤及8种胰腺肿瘤细胞系中细胞间黏附分子-1（ICAM-1，CD54）的表达情况。免疫组织化学显示，胰腺肿瘤细胞中ICAM-1阳性细胞的比例各不相同，而正常胰腺组织（除内皮细胞有轻微反应外）未被染色。我们对8种不同的胰腺肿瘤细胞系研究了细胞表面ICAM-1分子的存在情况及ICAM-1 mRNA的表达情况。其中3种细胞系（Capan-1、Capan-2、QGP-1）组成性表达ICAM-1。在4种ICAM-1阴性的胰腺癌细胞系中，通过在炎性细胞因子存在的情况下培养细胞，有可能诱导ICAM-1显著表达，而有一种细胞系818-4仍为ICAM-1阴性。结果显示，不同细胞系对IFN-γ、TNF-α或IL-1β处理的反应性各不相同，这表明在转录和转录后水平调节ICAM-1表达的机制很复杂。有趣的是，胰腺肿瘤细胞会释放ICAM-1，因为在细胞培养上清液中检测到了可溶性sICAM-1。与正常血清相比，胰腺癌患者血清中sICAM-1的平均水平升高了2倍。
