Bhat N M, Bieber M M, Teng N N
Department of Gynecology and Obstetrics, Stanford University, CA 94305.
J Immunol Methods. 1993 Feb 3;158(2):277-80. doi: 10.1016/0022-1759(93)90224-u.
We describe a discontinuous triple density gradient to obtain a 25-fold enrichment of nucleated red blood cells from mononuclear cells, granulocytes, and mature red blood cells in a single centrifugation step.
我们描述了一种不连续的三重密度梯度,可在单次离心步骤中从单核细胞、粒细胞和成熟红细胞中获得25倍富集的有核红细胞。