Wojcik B E, Nothias F, Lazar M, Jouin H, Nicolas J F, Peschanski M
Institut Pasteur, Centre National de Recherche Scientifique, Paris, France.
Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1305-9. doi: 10.1073/pnas.90.4.1305.
A replication-defective retrovirus was used to introduce the marker gene nlsLacZ into the murine embryonal carcinoma (EC) cell line PCC7-S-aza-R-1009. Undifferentiated EC cells were implanted into the central nervous system of adult rats. One month later, the grafted cells continued to express the nlsLacZ gene. Immunohistochemical analysis demonstrated the presence of EC-derived neurons. These neurons were capable of expressing tyrosine hydroxylase and extended neurites into the host parenchyma. EC-derived glial cells could not be detected. There was no evidence of tumorigenicity. These results demonstrate the utility of EC cells for introduction of exogenous gene products into the central nervous system in experimental models of gene therapy.
一种复制缺陷型逆转录病毒被用于将标记基因nlsLacZ导入小鼠胚胎癌细胞系PCC7-S-aza-R-1009。未分化的胚胎癌细胞被植入成年大鼠的中枢神经系统。一个月后，移植的细胞继续表达nlsLacZ基因。免疫组织化学分析证明存在胚胎癌衍生的神经元。这些神经元能够表达酪氨酸羟化酶并将神经突延伸到宿主实质中。未检测到胚胎癌衍生的胶质细胞。没有致瘤性的证据。这些结果证明了胚胎癌细胞在基因治疗实验模型中向中枢神经系统引入外源基因产物的实用性。
