Pfeiffer S E, Jakob H, Mikoshiba K, Dubois P, Guenet J L, Nicolas J F, Gaillard J, Chevance G, Jacob F
J Cell Biol. 1981 Jan;88(1):57-66. doi: 10.1083/jcb.88.1.57.
A line of embryonal carcinoma cells, PCC7-S, established in vitro from a spontaneous testicular teratocarcinoma, has been studied. Upon removing the cells from a low density monolayer culture system and permitting the cells to form aggregates in suspension, we observed a change of several physical and biochemical parameters: (a) reduction in average cell volume, (b) blockage and accumulation of cells in G1, (c) rise in secreted protease activity, (d) rise in acetylcholinesterase and choline acetyltransferase activities, and (e) disappearance of embryonic antigen F9. Although PCC7 aggregates did not undergo substantial morphological changes while suspended, when aggregates 4 or more days old were allowed to attach to plastic tissue culture dishes, substantial neurite outgrowth occurred over the next 1-3 d. This process was markedly enhanced by the addition to the growth medium of carboxymethylcellulose and inhibitors of DNA synthesis. Transmission electron microscopy disclosed a neurite ultrastructure consistent with that of neuronal processes. A veratridine-stimulated, tetrodotoxin-blocked sodium influx of 100 nmol/min per mg protein was also observed in these differentiated surface cultures. This cell line is discussed in terms of its utility for the study of early events leading to a commitment to cellular differentiation, as well as for the investigation of terminal differentiation to cholinergic neurons.
对一株从自发性睾丸畸胎瘤体外建立的胚胎癌细胞系PCC7-S进行了研究。将细胞从低密度单层培养系统中取出，使其在悬浮状态下形成聚集体后，我们观察到几个物理和生化参数发生了变化：(a)平均细胞体积减小；(b)细胞在G1期阻滞和积累；(c)分泌的蛋白酶活性升高；(d)乙酰胆碱酯酶和胆碱乙酰转移酶活性升高；(e)胚胎抗原F9消失。虽然PCC7聚集体在悬浮时未发生明显的形态变化，但当4天或更久的聚集体附着于塑料组织培养皿时，在接下来的1-3天内会出现大量神经突生长。向生长培养基中添加羧甲基纤维素和DNA合成抑制剂可显著增强这一过程。透射电子显微镜显示神经突超微结构与神经元突起一致。在这些分化的表面培养物中还观察到，藜芦碱刺激、河豚毒素阻断的钠内流为每毫克蛋白质每分钟100纳摩尔。本文讨论了该细胞系在研究导致细胞分化的早期事件以及胆碱能神经元终末分化方面的应用价值。
