Paulin D, Jakob H, Jacob F, Weber K, Osborn M
Differentiation. 1982;22(2):90-9. doi: 10.1111/j.1432-0436.1982.tb01231.x.
Teratocarcinoma differentiation has been studied using sera specific for each of the five intermediate filament (IF) classes. These antibodies distinguish cells of epithelial, muscle, neural, astrocytic, and mesenchymal origin. In embryoid bodies, derived from embryo transplants and obtained in the ascitic fluid by transplantation of teratocarcinoma, the cells of the inner cellular mass did not express any of these intermediate filament types while the outer cells expressed cytokeratin. Intermediate filament expression in the embryoid body thus appears analogous to that in the blastocyst and differs from that in embryonal carcinoma (EC) lines. Twelve EC lines have now been shown to express vimentin although in some EC lines not all cells express vimentin. Other established permanent differentiated cell lines, derived from EC lines in vitro or from tumors in vivo, have been characterized with respect to the type of IF they contain. The distribution of different IF types has been examined in EC cells induced to differentiate by addition of retinoic acid. The proportion of cells expressing each type of intermediate filament appears to depend on the EC cell line used, on the inducing agent, and on the length of treatment. Thus, for instance, F9 cells express cytokeratin, PCC3 derivatives express vimentin, many 1009 derivatives express either glial fibrillar acidic protein (GFA) or neurofilament proteins. Overall the results obtained are in excellent agreement with emerging principles of intermediate filament expression during embryonic differentiation, thus emphasizing the potential use of the various EC lines to study differentiation in culture.
利用针对五种中间丝（IF）类别的每种的特异性血清，对畸胎癌分化进行了研究。这些抗体可区分上皮、肌肉、神经、星形胶质细胞和间充质来源的细胞。在由胚胎移植产生并通过畸胎癌移植在腹水中获得的胚状体中，内细胞团的细胞不表达任何这些中间丝类型，而外层细胞表达细胞角蛋白。因此，胚状体中的中间丝表达似乎类似于囊胚中的表达，并且不同于胚胎癌（EC）细胞系中的表达。现已证明12种EC细胞系表达波形蛋白，尽管在一些EC细胞系中并非所有细胞都表达波形蛋白。其他已建立的永久分化细胞系，源自体外的EC细胞系或体内的肿瘤，已根据它们所含的IF类型进行了表征。已在通过添加视黄酸诱导分化的EC细胞中检查了不同IF类型的分布。表达每种中间丝类型的细胞比例似乎取决于所使用的EC细胞系、诱导剂以及处理时间的长短。因此，例如，F9细胞表达细胞角蛋白，PCC3衍生物表达波形蛋白，许多1009衍生物表达胶质纤维酸性蛋白（GFA）或神经丝蛋白。总体而言，所获得的结果与胚胎分化过程中中间丝表达的新出现的原则非常一致，从而强调了利用各种EC细胞系在培养中研究分化的潜力。
