Dumbell K, Richardson M
Department of Medical Microbiology, University of Cape Town, South Africa.
Arch Virol. 1993;128(3-4):257-67. doi: 10.1007/BF01309438.
Isolates of poxviruses were made from thirteen of eighteen specimens of scabs taken from pox lesions on buffaloes in five different districts of Maharashtra State, India, between December, 1985 and February, 1987. The biological characters of twelve of the isolates resembled those of the Hissar strain of buffalopox virus; the thirteenth isolate appeared to be vaccinia. The Hin dIII restriction profiles of DNA from all 13 isolates and from the Hissar strain were typical of those given by vaccinia strains. DNA from all twelve Maharashtra buffalopox (BPV) isolates gave identical profiles with each of three additional endonucleases; these viruses appear to be repeated isolations of a single strain of BPV. The DNA profile of this strain was not the same as that of the Hissar strain of BPV and both could readily be distinguished from each of the three strains of vaccinia virus which had been used in India. The thirteenth Maharashtra isolate was indistinguishable from vaccinia in its biological properties, but the restriction profile of its DNA differed from those of three vaccinia strains and the BPV isolates. These observations, made 6-8 years after cessation of smallpox vaccination indicate that BPV is an emerging enzootic virus and is a subspecies of vaccinia virus.
1985年12月至1987年2月期间，从印度马哈拉施特拉邦五个不同地区水牛痘疹痂皮的18个样本中，分离出了13株痘病毒。其中12株分离株的生物学特性与水牛痘病毒希萨尔株相似；第13株分离株似乎是牛痘病毒。所有13株分离株以及希萨尔株的DNA经Hin dIII酶切后的图谱，均为牛痘病毒株的典型图谱。来自马哈拉施特拉邦的所有12株水牛痘（BPV）分离株，经另外三种核酸内切酶酶切后，均呈现相同的图谱；这些病毒似乎是同一株BPV的重复分离株。该毒株的DNA图谱与BPV希萨尔株不同，且二者均能轻易地与印度使用过的三种牛痘病毒株区分开来。第13株马哈拉施特拉邦分离株在生物学特性上与牛痘病毒无法区分，但其DNA的酶切图谱与三种牛痘病毒株及BPV分离株不同。这些观察结果是在天花疫苗接种停止6 - 8年后得出的，表明BPV是一种新出现的地方流行性病毒，是牛痘病毒的一个亚种。
