Del Giudice G, Gervaix A, Costantino P, Wyler C A, Tougne C, de Graeff-Meeder E R, van Embden J, van der Zee R, Nencioni L, Rappuoli R
Department of Pathology, University of Geneva, Switzerland.
J Immunol. 1993 Mar 1;150(5):2025-32.
To investigate whether and in which proportion normal individuals experience a priming to microbial heat shock proteins (hsp), the presence of antibodies to two mycobacterial hsp was tested in serum sample from 2- to 4-mo-old children before and at different times after vaccination with the trivalent vaccine against tetanus, diphtheria, and pertussis (DTP). We show that 88.9% of infants vaccinated with DTP developed antibody responses to mycobacterial hsp. Such a response was due to the whole-cell pertussis component of the vaccine, because it was not observed in infants receiving an acellular pertussis vaccine. Antibodies and cells reactive to the mycobacterial 65-kDa hsp were also found in mice immunized with DTP. Interestingly, whole-cell pertussis vaccine-induced anti-hsp antibodies cross-reacted with the Escherichia coli GroEL hsp, and at a some extent with the human 60-kDa hsp, belonging to the same hsp family. These data suggest that priming of the immune system to hsp is a common phenomenon occurring very early in life.
为了研究正常个体是否以及以何种比例对微生物热休克蛋白（hsp）产生启动作用，我们检测了2至4月龄儿童在接种破伤风、白喉和百日咳三联疫苗（DTP）之前以及接种后不同时间的血清样本中针对两种分枝杆菌hsp的抗体。我们发现，88.9%接种DTP的婴儿对分枝杆菌hsp产生了抗体反应。这种反应是由于疫苗中的全细胞百日咳成分引起的，因为在接种无细胞百日咳疫苗的婴儿中未观察到这种反应。在用DTP免疫的小鼠中也发现了对分枝杆菌65-kDa hsp有反应的抗体和细胞。有趣的是，全细胞百日咳疫苗诱导的抗hsp抗体与大肠杆菌GroEL hsp发生交叉反应，并在一定程度上与属于同一hsp家族的人60-kDa hsp发生交叉反应。这些数据表明，免疫系统对hsp的启动是生命早期非常普遍的现象。
