Qazi Khaleda Rahman, Qazi Mousumi Rahman, Julián Esther, Singh Mahavir, Abedi-Valugerdi Manuchehr, Fernández Carmen
Department of Immunology, Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.
Infect Immun. 2005 Nov;73(11):7687-96. doi: 10.1128/IAI.73.11.7687-7696.2005.
During stress conditions, such as infection, the synthesis of heat shock proteins (HSPs) in microorganisms is upregulated. Since a high degree of homology exists within each HSP family, we postulated that exposure to microorganisms could prime the immune system for evolutionarily diverse HSPs. We tested this hypothesis by priming mice with three microorganisms, namely, Mycobacterium bovis BCG, Mycobacterium vaccae, and Chlamydia pneumoniae. After this, mice received a dose of the various HSPs. We found that BCG and M. vaccae but not C. pneumoniae primed the immune system for the induction of secondary immunoglobulin G (IgG) responses to most of the HSPs tested. Analysis of the IgG1 and IgG2a profile and gamma interferon production induced against the HSPs revealed the induction of a mixture of responses. We also observed that sera from mice treated with M. vaccae and HSP70 were cross-reactive, but no antibody complexes were observed in their kidneys, which frequently are targets for autoantibody reactions. Our findings add further support for the use of HSPs as effective vaccine adjuvants.
在应激条件下，如感染时，微生物中热休克蛋白（HSPs）的合成会上调。由于每个HSP家族内部存在高度同源性，我们推测接触微生物可能会使免疫系统对进化上不同的HSPs产生预刺激作用。我们通过用三种微生物，即牛分枝杆菌卡介苗、母牛分枝杆菌和肺炎衣原体对小鼠进行预刺激来验证这一假设。在此之后，小鼠接受不同剂量的各种HSPs。我们发现卡介苗和母牛分枝杆菌能使免疫系统对大多数测试的HSPs诱导产生二次免疫球蛋白G（IgG）反应，而肺炎衣原体则不能。对针对HSPs诱导产生的IgG1和IgG2a谱以及γ干扰素产生的分析揭示了多种反应的诱导。我们还观察到，用母牛分枝杆菌和HSP70处理的小鼠血清具有交叉反应性，但在其肾脏中未观察到抗体复合物，而肾脏通常是自身抗体反应的靶器官。我们的研究结果进一步支持了将HSPs用作有效疫苗佐剂的观点。
